Mouse CRISPR Knockout Pooled Library A+B(1 vector system)

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Mouse CRISPR Knockout Pooled Library A+B(1 vector system)

Mouse CRISPR Knockout Pooled Library A+B(1 vector system)

Catalog#： LIBR-M001AB-LV1000 LIBR-M001AB-LV5000 LIBR-M001AB-LV10000

Size ： 1*10^8TU 5*10^8TU 1*10^9TU

Instruction：

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Detailed Product Information Recommended Companion Products
Ubigene's CRISPR Library Virus is conducted by utilizing CRISPR iScreen™. Ubigene's Library Virus with high titer is obtained by firstly obtaining Library Plasmid with high coverage and good uniformity, then packaging the virus using Lentiviral Packaging Kit (#YK-LVP-20), collecting supernatant and eventually concentrating. Ubigene's CRISPR Library Virus can be directly used for the construction of CRISPR library stable cell pool, directly omitting the tedious and complex library plasmid amplification process.
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Product Information

Product Name
Mouse CRISPR Knockout Pooled Library A+B(1 vector system)
Organism
Mouse
Library Type
Knockout Library
Plasmid System
Single-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
20611
gRNA Number
130209
Non-targeting gRNA Number
1000
Label
Puro
Vector Formula
lentiCRISPR v2 vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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CRISPR Library Screen Service
Provides one-stop solutions for CRISPR-KO, CRISPRa, CRISPRi library screen from high-throughput sgRNA library construction, virus packaging, cell infection, drug screening, NGS sequencing to data analysis, etc. Various deliverables fulfill different research needs.
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Cas9 Stable Cell Line
The Cas9 cell lines in our cell bank can stably express Cas9 protein. So gene knockout can be achieved by transfecting gRNA. Simultaneously transfecting gRNA and donor DNA can achieve gene knock-in/point mutation, effectively improving experimental efficiency.
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